Ness-Greenstein R B, Rosenberg M, Doyle R J, Kaplan N
Maurice and Gabriela Goldschleger School of Dental Medicine, Tel-Aviv University, Israel.
FEMS Microbiol Lett. 1995 Jan 1;125(1):71-5. doi: 10.1111/j.1574-6968.1995.tb07337.x.
In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5 alpha cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized component(s) on the transformant outer surface.
为了确定粘质沙雷氏菌的疏水表面特性是否能够转移至大肠杆菌，用来自粘质沙雷氏菌RZ的DNA片段转化了大肠杆菌DH5α细胞。对1500个大肠杆菌转化子进行了十六烷和聚苯乙烯粘附性筛选。一个转化子对十六烷液滴的粘附性增强，并且在硫酸铵存在的情况下其聚集动力学发生了改变。蛋白质免疫印迹显示，针对粘质沙雷氏菌RZ产生的抗体识别该转化子外表面的成分。
