来自粗球孢子菌的精氨酸酶编码基因（arg）的分离与鉴定。

Isolation and characterization of the arginase-encoding gene (arg) from Coccidioides immitis.

作者信息

Pan S, Zhang M, Cole G T

机构信息

Department of Botany, University of Texas, Austin 78713.

出版信息

Gene. 1995 Feb 27;154(1):115-8. doi: 10.1016/0378-1119(94)00837-i.

DOI:10.1016/0378-1119(94)00837-i

PMID:7867937

Abstract

The arginase (ARG)-encoding gene (arg) of Coccidioides immitis, a human fungal pathogen, was cloned and sequenced. Both the genomic and cDNA sequences are provided. The transcription start point and poly(A) sites were confirmed. The arg gene, which was located on chromosome II of C. immitis by Southern hybridization, is a single-copy gene with two introns and a 966-bp ORF which translates a 322-aa protein of 35.1 kDa. The deduced ARG protein showed 44% identity and 68% similarity to the Saccharomyces cerevisiae ARG.

摘要

对人类真菌病原体粗球孢子菌的精氨酸酶（ARG）编码基因（arg）进行了克隆和测序。提供了基因组序列和cDNA序列。确定了转录起始点和聚腺苷酸化位点。通过Southern杂交将arg基因定位在粗球孢子菌的II号染色体上，它是一个单拷贝基因，有两个内含子和一个966 bp的开放阅读框，可翻译出一个35.1 kDa的322个氨基酸的蛋白质。推导的ARG蛋白与酿酒酵母ARG的同一性为44%，相似性为68%。