Wyckoff E E, Pishko E J, Kirkland T N, Cole G T
Department of Botany, University of Texas, Austin, 78713, USA.
Gene. 1995 Aug 8;161(1):107-11. doi: 10.1016/0378-1119(95)00250-a.
The gene which encodes a previously described T-cell reactive protein (TCRP) of the human fungal pathogen Coccidioides immitis (Ci) was cloned and sequenced. Both the genomic and cDNA sequences were determined. The transcription start point was confirmed. The tcrP gene has three introns and a 1197-bp ORF which translates to a 399-amino-acid (aa) protein (45.2 kDa). The predicted protein has approx. 50% aa sequence identity and 70% similarity to mammalian 4-hydroxyphenylpyruvate dioxygenase (HPPD) proteins and mammalian F-antigens. Expression of the Ci tcrP in Escherichia coli resulted in production of a deep brown pigment, consistent with E. coli expression of the bacterial HPPD homolog from Shewanella colwelliana. The TCRP is likely the Ci form of HPPD.
编码人真菌病原体球孢子菌（Ci）先前描述的T细胞反应蛋白（TCRP）的基因被克隆并测序。确定了基因组序列和cDNA序列。确认了转录起始点。tcrP基因有三个内含子和一个1197 bp的开放阅读框（ORF），其编码一个399个氨基酸（aa）的蛋白质（45.2 kDa）。预测的蛋白质与哺乳动物4-羟基苯丙酮酸双加氧酶（HPPD）蛋白和哺乳动物F抗原的氨基酸序列同一性约为50%，相似性为70%。Ci tcrP在大肠杆菌中的表达导致产生深棕色色素，这与大肠杆菌表达来自希瓦氏菌的细菌HPPD同源物一致。TCRP可能是HPPD的Ci形式。
