Vega M, Castillo T, Retamales I, Las Heras J, Devoto L, Videla L A
Department of Cell Biology and Genetics, Faculty of Medicine, University of Chile.
Free Radic Biol Med. 1994 Dec;17(6):493-9. doi: 10.1016/0891-5849(94)90088-4.
The steroidogenic capacity and oxidative stress-related parameters of the human corpus luteum (CL) at different stages of the luteal phase were studied under basal and human chorionic gonadotropin (hCG)-stimulated conditions. Mid CL exhibited the maximal steroidogenic capacity, together with lower levels of glutathione and higher thiobarbituric acid reactants content, macrophage count, and superoxide dismutase (SOD) activity, compared to the late CL. Addition of hCG to luteal cell cultures led to a preferential increase in progesterone synthesis in the late CL compared to the mid CL, without changes in the oxidative stress-related parameters, except for the increased SOD activity found in the late CL. It is concluded that an oxidative stress condition is established in the mid CL, coinciding with the maximal steroidogenic capacity and macrophage infiltration of the organ, which may be of relevance as one of the major mechanisms initiating CL involution in the human.
在基础状态和人绒毛膜促性腺激素（hCG）刺激条件下，研究了黄体期不同阶段人黄体（CL）的类固醇生成能力和氧化应激相关参数。与晚期黄体相比，中期黄体表现出最大的类固醇生成能力，同时谷胱甘肽水平较低，硫代巴比妥酸反应物含量、巨噬细胞计数和超氧化物歧化酶（SOD）活性较高。在黄体细胞培养物中添加hCG后，与中期黄体相比，晚期黄体中的孕酮合成优先增加，氧化应激相关参数没有变化，但晚期黄体中的SOD活性增加。结论是，中期黄体中建立了氧化应激状态，这与该器官的最大类固醇生成能力和巨噬细胞浸润相一致，这可能是启动人类黄体退化的主要机制之一。
