Kasapović J, Pajović S B, Kanazir D T, Martinović J V
Laboratory for Molecular Biology and Endocrinology, Vinca Institute for Nuclear Sciences, Belgrade, Serbia, Yugoslavia.
J Endocrinol Invest. 1997 Apr;20(4):203-6. doi: 10.1007/BF03346903.
Activities of superoxide dismutases MnSOD and CuZnSOD were measured in appropriate subcellular fractions prepared from livers of intact and long-term gonadectomized (GX) rats of both sexes, and of GX female and male rats injected sc with a single dose of 5 micrograms estradiol benzoate (EB) or 2 mg progesterone (P). In female livers, MnSOD activity did not vary significantly during the estrous cycle, declined after gonadectomy in comparison to proestrus, and was steady in GX females treated with EB or P. The activity of CuZnSOD was lowered at proestrus and elevated after removal of the ovaries in comparison to proestrus value. EB suppressed, and P elevated CuZnSOD activity in GX females. In the liver of male rats, MnSOD was not affected by gonadectomy nor by EB and P treatments. CuZnSOD activity was reduced following orchiectomy and enhanced in GX males following treatment with P, while EB had no effect. These results suggest that P and EB modulate the activity of CuZnSOD and do not affect MnSOD in the rat liver. The modulatory effects are elicited by P in the males and by P and EB in the females.
在来源于完整的和长期去性腺（GX）的两性大鼠肝脏，以及皮下注射单剂量5微克苯甲酸雌二醇（EB）或2毫克孕酮（P）的去性腺雌性和雄性大鼠肝脏所制备的合适亚细胞组分中，测定了超氧化物歧化酶MnSOD和CuZnSOD的活性。在雌性大鼠肝脏中，MnSOD活性在动情周期中无显著变化，与动情前期相比，去性腺后降低，而在用EB或P处理的去性腺雌性大鼠中保持稳定。与动情前期值相比，CuZnSOD活性在动情前期降低，卵巢切除后升高。EB抑制去性腺雌性大鼠的CuZnSOD活性，而P则升高其活性。在雄性大鼠肝脏中，MnSOD不受去性腺以及EB和P处理的影响。睾丸切除后CuZnSOD活性降低，用P处理的去性腺雄性大鼠中CuZnSOD活性增强，而EB则无作用。这些结果表明，P和EB调节大鼠肝脏中CuZnSOD的活性，而不影响MnSOD。雄性中P以及雌性中P和EB引发调节作用。
