球三糖神经酰胺（Gb3）是志贺样毒素2e的一种替代性功能性受体。
Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e.
作者信息
Keusch G T, Jacewicz M, Acheson D W, Donohue-Rolfe A, Kane A V, McCluer R H
机构信息
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Boston, Massachusetts.
出版信息
Infect Immun. 1995 Mar;63(3):1138-41. doi: 10.1128/iai.63.3.1138-1141.1995.
Abstract
We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially uses globotetraosylceramide as a receptor but can also cause cytotoxicity by using globotriaosylceramide, the SLT2 receptor. We conclude that the differential cytotoxicity of SLT2e on HeLa and Vero cells is a function of both the receptor preference of the toxin and the specific glycolipid content of the target cells being used.
摘要
我们通过使用受体缺陷细胞和纯化糖脂的脂质体插入系统，重新研究了与猪水肿病相关的志贺样毒素变体SLT2e的结合特异性，据报道该毒素对Vero细胞的细胞毒性比对HeLa细胞更强。我们发现，SLT2e优先使用球四糖神经酰胺作为受体，但也可以通过使用球三糖神经酰胺（SLT2受体）引起细胞毒性。我们得出结论，SLT2e对HeLa细胞和Vero细胞的不同细胞毒性是毒素受体偏好和所用靶细胞特定糖脂含量共同作用的结果。
相似文献
1
Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e.球三糖神经酰胺（Gb3）是志贺样毒素2e的一种替代性功能性受体。
Infect Immun. 1995 Mar;63(3):1138-41. doi: 10.1128/iai.63.3.1138-1141.1995.
2
Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variants.II型志贺样毒素与II型志贺样毒素变体的糖脂受体特异性比较。
Infect Immun. 1990 Mar;58(3):611-8. doi: 10.1128/iai.58.3.611-618.1990.
3
Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide.志贺氏菌腹泻的发病机制。十一、从兔空肠和海拉细胞中分离出一种志贺氏菌毒素结合糖脂，并鉴定其为球三糖基神经酰胺。
J Exp Med. 1986 Jun 1;163(6):1391-404. doi: 10.1084/jem.163.6.1391.
4
Quantitation of the rabbit intestinal glycolipid receptor for Shiga toxin. Further evidence for the developmental regulation of globotriaosylceramide in microvillus membranes.志贺毒素兔肠道糖脂受体的定量分析。微绒毛膜中球三糖神经酰胺发育调控的进一步证据。
Gastroenterology. 1989 Aug;97(2):384-91. doi: 10.1016/0016-5085(89)90074-7.
5
Globotriaosylceramide (Gb3) content in HeLa cells is correlated to Shiga toxin-induced cytotoxicity and Gb3 synthase expression.人宫颈癌细胞系（HeLa细胞）中的球三糖神经酰胺（Gb3）含量与志贺毒素诱导的细胞毒性及Gb3合酶表达相关。
BMB Rep. 2009 May 31;42(5):310-4. doi: 10.5483/bmbrep.2009.42.5.310.
6
Globotetraosylceramide is recognized by the pig edema disease toxin.球四糖神经酰胺可被猪水肿病毒素识别。
J Biol Chem. 1989 Jul 25;264(21):12520-5.
7
Expression of Shiga toxin 2e glycosphingolipid receptors of primary porcine brain endothelial cells and toxin-mediated breakdown of the blood-brain barrier.原代猪脑内皮细胞中志贺毒素 2e 糖鞘脂受体的表达及其介导的血脑屏障破坏。
Glycobiology. 2013 Jun;23(6):745-59. doi: 10.1093/glycob/cwt013. Epub 2013 Feb 21.
8
Pathogenesis of Shigella diarrhea: XVII. A mammalian cell membrane glycolipid, Gb3, is required but not sufficient to confer sensitivity to Shiga toxin.志贺氏菌腹泻的发病机制：十七、一种哺乳动物细胞膜糖脂Gb3是赋予对志贺毒素敏感性所必需的，但并不充分。
J Infect Dis. 1994 Mar;169(3):538-46. doi: 10.1093/infdis/169.3.538.
9
Human milk contains the Shiga toxin and Shiga-like toxin receptor glycolipid Gb3.人乳中含有志贺毒素和志贺样毒素受体糖脂Gb3。
J Infect Dis. 1992 Oct;166(4):832-6. doi: 10.1093/infdis/166.4.832.
10
Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity.原发性人肾皮质上皮细胞（pHRCEpiCs）上的志贺毒素（Stx）结合糖鞘脂和 Stx 介导的细胞毒性。
Toxins (Basel). 2021 Feb 12;13(2):139. doi: 10.3390/toxins13020139.
引用本文的文献
1
Fabry Disease: Cardiac Implications and Molecular Mechanisms.法布里病：心脏影响与分子机制。
Curr Heart Fail Rep. 2024 Apr;21(2):81-100. doi: 10.1007/s11897-024-00645-1. Epub 2024 Jan 30.
2
Identification of two lipid phosphatases that regulate sphingosine-1-phosphate cellular uptake and recycling.鉴定两种调节鞘氨醇-1-磷酸细胞摄取和回收的脂质磷酸酶。
J Lipid Res. 2022 Jun;63(6):100225. doi: 10.1016/j.jlr.2022.100225. Epub 2022 May 11.
3
Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3.与糖鞘脂Gb3结合的凝集素的结构多样性
Front Mol Biosci. 2021 Jul 26;8:704685. doi: 10.3389/fmolb.2021.704685. eCollection 2021.
4
Lectin-Mediated Binding of Engineered to Cancer Cells.凝集素介导的工程化物质与癌细胞的结合。
Microorganisms. 2021 Jan 22;9(2):223. doi: 10.3390/microorganisms9020223.
5
Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome.志贺毒素介导的 EHEC 相关溶血尿毒综合征中发育中红细胞损伤的合理推测。
Toxins (Basel). 2020 Jun 4;12(6):373. doi: 10.3390/toxins12060373.
6
A genome-wide CRISPR/Cas9 screen reveals that the aryl hydrocarbon receptor stimulates sphingolipid levels.全基因组 CRISPR/Cas9 筛选揭示芳香烃受体可刺激神经鞘脂水平。
J Biol Chem. 2020 Mar 27;295(13):4341-4349. doi: 10.1074/jbc.AC119.011170. Epub 2020 Feb 6.
7
Structural Insights into Shiga Toxin (Stx) Glycosphingolipid Receptors of Porcine Renal Epithelial Cells and Inhibition of Stx-Mediated Cellular Injury Using Neoglycolipid-Spiked Glycovesicles.猪肾上皮细胞志贺毒素（Stx）糖鞘脂受体的结构洞察以及使用新糖脂加标糖囊泡抑制Stx介导的细胞损伤
Microorganisms. 2019 Nov 19;7(11):582. doi: 10.3390/microorganisms7110582.
8
Bimodal Response to Shiga Toxin 2 Subtypes Results from Relatively Weak Binding to the Target Cell.对志贺毒素 2 亚型的双模态反应源于与靶细胞的相对较弱结合。
Infect Immun. 2019 Nov 18;87(12). doi: 10.1128/IAI.00428-19. Print 2019 Dec.
9
LPS-primed CD11b leukocytes serve as an effective carrier of Shiga toxin 2 to cause hemolytic uremic syndrome in mice.脂多糖预刺激的 CD11b 白细胞可作为志贺毒素 2 的有效载体，导致小鼠溶血性尿毒症综合征。
Sci Rep. 2018 Mar 5;8(1):3994. doi: 10.1038/s41598-018-22327-4.
10
A Topographical Atlas of Shiga Toxin 2e Receptor Distribution in the Tissues of Weaned Piglets.断奶仔猪组织中志贺毒素2e受体分布的地形图集
Toxins (Basel). 2016 Nov 30;8(12):357. doi: 10.3390/toxins8120357.
本文引用的文献
1
One step high yield affinity purification of shiga-like toxin II variants and quantitation using enzyme linked immunosorbent assays.
Microb Pathog. 1993 Jan;14(1):57-66. doi: 10.1006/mpat.1993.1006.
2
Pathogenesis of Shigella diarrhea: XVII. A mammalian cell membrane glycolipid, Gb3, is required but not sufficient to confer sensitivity to Shiga toxin.志贺氏菌腹泻的发病机制：十七、一种哺乳动物细胞膜糖脂Gb3是赋予对志贺毒素敏感性所必需的，但并不充分。
J Infect Dis. 1994 Mar;169(3):538-46. doi: 10.1093/infdis/169.3.538.
3
Shiga and Shiga-like toxins.志贺毒素和类志贺毒素。
Microbiol Rev. 1987 Jun;51(2):206-20. doi: 10.1128/mr.51.2.206-220.1987.
4
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine.来自引起猪水肿病的大肠杆菌菌株的II型志贺样毒素变体的克隆与测序
J Bacteriol. 1988 Sep;170(9):4223-30. doi: 10.1128/jb.170.9.4223-4230.1988.
5
6
Globotetraosylceramide is recognized by the pig edema disease toxin.球四糖神经酰胺可被猪水肿病毒素识别。
J Biol Chem. 1989 Jul 25;264(21):12520-5.
7
Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2).从一株与志贺样毒素II（VT2）具有免疫相关性的人源大肠杆菌中纯化出一种Vero毒素及其某些特性。
Microb Pathog. 1989 Feb;6(2):113-22. doi: 10.1016/0882-4010(89)90014-4.
8
Binding of verocytotoxin 1 to its receptor is influenced by differences in receptor fatty acid content.
Biochemistry. 1992 Feb 11;31(5):1363-70. doi: 10.1021/bi00120a011.
Zotero 插件