Morris D J, Corbitt G, Crosdale E
North Manchester Virus Laboratory, Booth Hall Children's Hospital, Blackley, Manchester, UK.
J Virol Methods. 1994 Oct;49(3):367-70. doi: 10.1016/0166-0934(94)90152-x.
We analysed the specificity of a screening enzyme-linked immunosorbent assay for human immunodeficiency virus antibodies, and found that 18 of 1878 serum specimens (1.0%) from an open access clinic gave false reactive results. Introduction of true same-day testing therefore required the use of more than one assay before a reactive result could be reported. A testing strategy was devised with a 4 h turn-around time which gave accurate results for 11 positive and 119 negative specimens.
我们分析了一种用于检测人类免疫缺陷病毒抗体的筛查酶联免疫吸附测定法的特异性，发现来自一家开放式诊所的1878份血清样本中有18份（1.0%）给出了假阳性结果。因此，要实现真正的当日检测，就需要在报告阳性结果之前使用不止一种检测方法。我们设计了一种周转时间为4小时的检测策略，该策略对11份阳性样本和119份阴性样本都给出了准确结果。
