一种凝集法HIV-1 + 2抗体检测方法的评估

Evaluation of an agglutination HIV-1 + 2 antibody assay.

作者信息

Vercauteren G, Beelaert G, van der Groen G

机构信息

Institute of Tropical Medicine, Department of Microbiology, Antwerp, Belgium.

出版信息

J Virol Methods. 1995 Jan;51(1):1-8. doi: 10.1016/0166-0934(94)00092-u.

DOI:10.1016/0166-0934(94)00092-u

PMID:7730430

Abstract

Studies have shown that an HIV (HIV-PA) agglutination assay (Serodia) for the detection of antibody to human immunodeficiency virus (HIV) can be as sensitive and as specific as enzyme-linked immunosorbent assay (ELISA). However, since this HIV assay was designed to detect antibody to the HIV-1 virus, a substantial number of HIV-2 positive sera are missed by this assay. Since the HIV-2 has now been found throughout the world this test is becoming less suitable. The new HIV-1 + 2 assay version (HIV-1 + 2 PA) was evaluated in 300 sera, which contained 50 HIV-1, 40 HIV-2 and 10 HIV-1/HIV-2 antibody positive samples, and a sensitivity and specificity of 100% and 99%, respectively, was obtained. Whereas all HIV-2 positive sera were detected by the new HIV-1 + 2 version, 26% (13/50) were missed by the old version of the agglutination test. It is concluded that the HIV-1 + 2 PA assay is a promising instrument free assay which can be used for screening purposes in areas where both HIV-1 and HIV-2 are present.

摘要

研究表明，用于检测人类免疫缺陷病毒（HIV）抗体的HIV（HIV-PA）凝集试验（Serodia）与酶联免疫吸附测定（ELISA）一样灵敏且特异。然而，由于该HIV检测方法旨在检测针对HIV-1病毒的抗体，大量HIV-2阳性血清会被该检测方法遗漏。鉴于目前已在全球发现HIV-2，该检测方法越来越不合适。新的HIV-1 + 2检测版本（HIV-1 + 2 PA）在300份血清中进行了评估，其中包含50份HIV-1、40份HIV-2和10份HIV-1/HIV-2抗体阳性样本，获得的灵敏度和特异性分别为100%和99%。新的HIV-1 + 2版本检测出了所有HIV-2阳性血清，而旧版本的凝集试验遗漏了26%（13/50）的样本。结论是，HIV-1 + 2 PA检测是一种有前景的无需仪器的检测方法，可用于HIV-1和HIV-2均存在地区的筛查。