非典型纤维黄色瘤中的非整倍体：通过图像分析对10例病例的DNA含量进行定量分析。

Aneuploidy in atypical fibroxanthoma: DNA content quantification of 10 cases by image analysis.

作者信息

Michie B A, Reid R P, Fallowfield M E

机构信息

Department of Pathology, Glasgow Royal Infirmary, Scotland.

出版信息

J Cutan Pathol. 1994 Oct;21(5):404-7. doi: 10.1111/j.1600-0560.1994.tb00280.x.

DOI:10.1111/j.1600-0560.1994.tb00280.x

PMID:7868751

Abstract

It has recently been reported that atypical fibroxanthoma (AFX) is a predominantly diploid lesion in contrast to malignant fibrous hystiocytoma (MFH) which is usually aneuploid. To test this hypothesis, DNA content quantification was undertaken on Feulgen-stained cytology and tissue section preparations from 10 cases of AFX by image analysis. The large atypical cells which characterize AFX were aneuploid in each case. Smaller spindle-shaped cells found in this lesion were diploid. The results suggest that AFX is indistinguishable from MFH by DNA content estimation and highlight an advantage of image analysis over flow cytometry.

摘要

最近有报道称，非典型纤维黄色瘤（AFX）主要是二倍体病变，而恶性纤维组织细胞瘤（MFH）通常是非整倍体。为验证这一假说，通过图像分析对10例AFX的福尔根染色细胞学和组织切片标本进行了DNA含量定量分析。AFX特征性的大非典型细胞在每例中均为非整倍体。该病变中发现的较小梭形细胞为二倍体。结果表明，通过DNA含量评估，AFX与MFH无法区分，同时突出了图像分析相对于流式细胞术的一个优势。