McCammon J R
Infect Immun. 1976 Sep;14(3):811-5. doi: 10.1128/iai.14.3.811-815.1976.
Antibody against reovirus type I must partially purified and conjugated with fluorescein isothiocyanate (FITC). The FITC-labeled antibody was then conjugated with 125I. A gamma globulin fraction of normal sera was similarly labeled with FITC followed by a 131I label. The FITC + 125I-labeled immune reagent was then mixed on an equal protein basis with the FITC + 131I control reagent. This mixture was used to stain acetone-fixed reovirus-infected cover slips. After staining, the cover slips were examined by fluorescence microscopy. Infected cover slips demonstrated characteristic reovirus immunofluorescence, whereas uninfected cover slips were negative. After visual examination, the cover slips were placed in tubes and counted in a two-channel gamma analyzer. By comparing the quantitative isotope data with the qualtitative information from immunofluorescence on a single preparation, it was possible to correlate antigen production sites with quantitative production values.
抗I型呼肠孤病毒抗体必须进行部分纯化，并与异硫氰酸荧光素（FITC）偶联。然后将FITC标记的抗体与125I偶联。正常血清的γ球蛋白部分同样先用FITC标记，然后用131I标记。然后将FITC + 125I标记的免疫试剂与FITC + 131I对照试剂按相同蛋白质含量混合在一起。该混合物用于对经丙酮固定的感染呼肠孤病毒的盖玻片进行染色。染色后，通过荧光显微镜检查盖玻片。感染的盖玻片呈现出特征性的呼肠孤病毒免疫荧光，而未感染的盖玻片为阴性。肉眼检查后，将盖玻片放入试管中，在双通道γ分析仪中进行计数。通过将定量同位素数据与来自单一制剂免疫荧光的定性信息进行比较，有可能将抗原产生部位与定量产生值相关联。
