辣根过氧化物酶标记抗体用于呼肠孤病毒抗原的光镜和电镜定位
Use of horseradish peroxidase-labeled antibody for light and electron microscope localization of reovirus antigen.
作者信息
Ubertini T, Wilkie B N, Noronha F
出版信息
Appl Microbiol. 1971 Mar;21(3):534-8. doi: 10.1128/am.21.3.534-538.1971.
Abstract
Horseradish peroxidase-labeled antibody was used for light and electron microscopic localization of reovirus antigen in tissue culture. Reaction product in infected cells was easily detected in the cytoplasm, and the procedure was as sensitive as the fluorescent-antibody technique. At the electron microscopic level, infected and enzyme-labeled antibody-treated cells showed accumulations of reaction product at the sites of viral replication and around the viral particles. Reaction product was not detected in unstained infected cells, in stained uninfected cells, or in cells infected with an unrelated virus.
摘要
辣根过氧化物酶标记抗体用于在组织培养中对呼肠孤病毒抗原进行光镜和电镜定位。感染细胞中的反应产物在细胞质中易于检测到，该方法与荧光抗体技术一样灵敏。在电子显微镜水平上，感染并经酶标记抗体处理的细胞在病毒复制位点和病毒颗粒周围显示出反应产物的积累。在未染色的感染细胞、染色的未感染细胞或感染无关病毒的细胞中未检测到反应产物。
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