Hotsubo T, Nagata N, Shimada M, Yoshida K, Fujinaga K, Chiba S
Department of Pediatrics, School of Medicine, Sapporo Medical University, Hokkaido, Japan.
Microbiol Immunol. 1994;38(10):809-11. doi: 10.1111/j.1348-0421.1994.tb01862.x.
Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.
