Igarashi A, Tanaka M, Morita K, Takasu T, Ahmed A, Ahmed A, Akram D S, Waqar M A
Department of Virology, Nagasaki University, Japan.
Microbiol Immunol. 1994;38(10):827-30. doi: 10.1111/j.1348-0421.1994.tb01866.x.
Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.
1992年2月至8月间收集的24份脑脊液（CSF）标本经逆转录酶聚合酶链反应（RT-PCR）检测，在8份标本中检测到西尼罗河（WN）病毒基因组序列，在1份标本中检测到日本脑炎（JE）病毒基因组序列。这些结果与脑脊液的IgM-ELISA数据相结合表明，巴基斯坦卡拉奇相当一部分急性脑炎病例是由WN病毒感染引起的，而JE病毒引起的病例占比很小。
