Lennette D A, Emmons R W, Lennette E H
J Clin Microbiol. 1976 Aug;2(2):81-4.
Mumps and its complications, particularly meningoencephalitis, is an important disease problem, and more rapid diagnostic methods are desirable. A study was made of immunofluorescence methods for the early detection of mumps virus isolated in cell cultures, or adsorbed directly from clinical specimens onto guinea pig erythrocytes. A specific diagnosis could be made in hours to 2 or 3 days utilizing immunofluorescence methods, in contrast to about 6 days by standard methods. Details of the direct immunofluorescence methods are presented, to encourage wider application in clinical virology laboratories.
腮腺炎及其并发症,尤其是脑膜脑炎,是一个重要的疾病问题,因此需要更快速的诊断方法。本研究针对免疫荧光法进行了探究,该方法用于早期检测在细胞培养物中分离出的腮腺炎病毒,或直接从临床标本吸附到豚鼠红细胞上的腮腺炎病毒。与标准方法大约需要6天相比,利用免疫荧光法可在数小时至2至3天内做出明确诊断。本文介绍了直接免疫荧光法的详细信息,以鼓励其在临床病毒学实验室中得到更广泛的应用。