通过免疫荧光法快速诊断腮腺炎病毒感染
Rapid diagnosis of mumps virus infections by immunofluorescence methods.
作者信息
Lennette D A, Emmons R W, Lennette E H
出版信息
J Clin Microbiol. 1976 Aug;2(2):81-4.
Abstract
Mumps and its complications, particularly meningoencephalitis, is an important disease problem, and more rapid diagnostic methods are desirable. A study was made of immunofluorescence methods for the early detection of mumps virus isolated in cell cultures, or adsorbed directly from clinical specimens onto guinea pig erythrocytes. A specific diagnosis could be made in hours to 2 or 3 days utilizing immunofluorescence methods, in contrast to about 6 days by standard methods. Details of the direct immunofluorescence methods are presented, to encourage wider application in clinical virology laboratories.
摘要
腮腺炎及其并发症，尤其是脑膜脑炎，是一个重要的疾病问题，因此需要更快速的诊断方法。本研究针对免疫荧光法进行了探究，该方法用于早期检测在细胞培养物中分离出的腮腺炎病毒，或直接从临床标本吸附到豚鼠红细胞上的腮腺炎病毒。与标准方法大约需要6天相比，利用免疫荧光法可在数小时至2至3天内做出明确诊断。本文介绍了直接免疫荧光法的详细信息，以鼓励其在临床病毒学实验室中得到更广泛的应用。
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本文引用的文献
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THE DIAGNOSIS OF RABIES BY FLUORESCENT ANTIBODY METHOD (FRA) EMPLOYING IMMUNE HAMSTER SERUM.采用免疫仓鼠血清的荧光抗体法（FRA）诊断狂犬病
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Serologic responses to para-influenza viruses in patients with mumps virus injection.腮腺炎病毒注射患者对副流感病毒的血清学反应。
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Application of the method of immunofluorescence for rapid diagnosis of mumps virus.免疫荧光法在腮腺炎病毒快速诊断中的应用。
J Hyg Epidemiol Microbiol Immunol. 1970;14(1):19-26.
4
Laboratory diagnosis of mumps by immunofluorescence on experimentally inoculated cell cultures.通过对实验接种的细胞培养物进行免疫荧光检测来进行腮腺炎的实验室诊断。
Scand J Infect Dis. 1972;4(2):105-8. doi: 10.3109/inf.1972.4.issue-2.07.
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Rapid diagnosis of meningoencephalitis, encephalitis. Immunofluorescent examination of fresh and in vitro cultured cerebrospinal fluid cells.脑膜脑炎、脑炎的快速诊断。新鲜及体外培养的脑脊液细胞的免疫荧光检查。
Neurology. 1974 Feb;24(2):143-8. doi: 10.1212/wnl.24.2.143.
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Immunofluorescence methods for detection of influenza and mumps antibodies using infected shedded allantoic cells and impression smears of organ cultures as the sources of antigens.使用感染后脱落的尿囊细胞和器官培养物的印片作为抗原来源，检测流感和腮腺炎抗体的免疫荧光方法。
Arch Gesamte Virusforsch. 1973;41(4):354-9. doi: 10.1007/BF01250207.
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The examination of cerebrospinal fluid cells by fluorescent antibody staining to detect mumps antigen.通过荧光抗体染色检测脑脊液细胞中的腮腺炎抗原。
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