Chaumont F, Schanck A N, Blum J J, Opperdoes F R
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Mol Biochem Parasitol. 1994 Oct;67(2):321-31. doi: 10.1016/0166-6851(94)00141-3.
Metabolic studies on Phytomonas sp. isolated from the lactiferous tubes of the latex-bearing spurge Euphorbia characias indicate that glucose is the preferred energy and carbon substrate during logarithmic growth. In stationary phase cells glucose consumption was dramatically reduced. Glucose consumption and end-product formation were measured on logarithmically growing cells, both under aerobic (air and 95% O2/5% CO2) and anaerobic (95% N2/5% CO2 and 100% N2) conditions. The rate of glucose consumption slightly increased under anaerobic conditions indicating that Phytomonas lacks a 'reverse Pasteur' effect contrary to the situation encountered in Leishmania major. Major end-products of glucose catabolism under aerobic conditions, detected by enzymatic and NMR measurements, were acetate, ethanol and carbon dioxide and under anaerobic conditions ethanol, glycerol and carbon dioxide. Smaller amounts of pyruvate, succinate, L-malate, L-lactate, phosphoenolpyruvate, alanine and aspartate were also detected.
对从产胶大戟（Euphorbia characias）的乳汁管中分离出的植滴虫（Phytomonas sp.）进行的代谢研究表明，在对数生长期，葡萄糖是首选的能量和碳源底物。在稳定期，细胞对葡萄糖的消耗显著减少。在有氧（空气和95% O₂/5% CO₂）和无氧（95% N₂/5% CO₂和100% N₂）条件下，对对数生长期的细胞测量了葡萄糖消耗和终产物形成。在无氧条件下，葡萄糖消耗速率略有增加，这表明植滴虫缺乏“反向巴斯德”效应，这与杜氏利什曼原虫（Leishmania major）的情况相反。通过酶法和核磁共振测量检测到，在有氧条件下，葡萄糖分解代谢的主要终产物是乙酸盐、乙醇和二氧化碳；在无氧条件下，是乙醇、甘油和二氧化碳。还检测到少量的丙酮酸、琥珀酸盐、L-苹果酸、L-乳酸、磷酸烯醇丙酮酸、丙氨酸和天冬氨酸。
