Darling T N, Davis D G, London R E, Blum J J
Department of Physiology, Duke University Medical Center, Durham, NC 27710.
Proc Natl Acad Sci U S A. 1987 Oct;84(20):7129-33. doi: 10.1073/pnas.84.20.7129.
Leishmania braziliensis panamensis promastigotes were incubated with glucose as the sole carbon source. About one-fifth of the glucose consumed under aerobic conditions was oxidized to CO2. Nuclear magnetic resonance studies with [1-13C]glucose showed that the other products released were succinate, acetate, alanine, pyruvate, and lactate. Under anaerobic conditions, lactate output increased, glycerol became a major product, and, surprisingly, glucose consumption decreased. Enzymatic assays showed that the lactate formed was D(-)-lactate. The release of alanine during incubation with glucose as the sole carbon source suggested that appreciable proteolysis occurred, consistent with our observation that a large amount of ammonia was released under these conditions. The discoveries that D-lactate is a product of L. braziliensis glucose catabolism, that glycerol is produced under anaerobic conditions, and that the cells exhibit a "reverse" Pasteur effect open the way for detailed studies of the pathways of glucose metabolism and their regulation in this organism.
将巴西利什曼原虫巴拿马亚种前鞭毛体与葡萄糖作为唯一碳源一起培养。在有氧条件下消耗的葡萄糖中约五分之一被氧化为二氧化碳。用[1-13C]葡萄糖进行的核磁共振研究表明，释放的其他产物是琥珀酸、乙酸、丙氨酸、丙酮酸和乳酸。在厌氧条件下，乳酸产量增加，甘油成为主要产物，令人惊讶的是，葡萄糖消耗减少。酶分析表明形成的乳酸是D(-)-乳酸。以葡萄糖作为唯一碳源培养期间丙氨酸的释放表明发生了明显的蛋白质水解，这与我们在这些条件下观察到大量氨释放的结果一致。D-乳酸是巴西利什曼原虫葡萄糖分解代谢的产物、甘油在厌氧条件下产生以及细胞表现出“反向”巴斯德效应这些发现为详细研究该生物体中葡萄糖代谢途径及其调节开辟了道路。
