Rainey P M, MacKenzie N E
Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510.
Mol Biochem Parasitol. 1991 Apr;45(2):307-15. doi: 10.1016/0166-6851(91)90099-r.
The major products of glucose metabolism were determined for amastigotes and promastigotes of Leishmania (mexicana) pifanoi under aerobic and anaerobic conditions using carbon-13 nuclear magnetic resonance. Under aerobic conditions, the major products for both forms were carbon dioxide, succinate, malate, acetate and alanine. Succinate was the dominant metabolite of promastigotes, whereas acetate and alanine were most abundant with amastigotes. Under anaerobic conditions, promastigotes produced glycerol as the dominant metabolite, along with lesser amounts of succinate, acetate and alanine; acetate and alanine remained major metabolites in amastigotes, with an increase in the relative amount of succinate and the production of some glycerol. Promastigotes generated carbon dioxide at a 5-fold greater rate than amastigotes under aerobic conditions, but this rate was reduced by more than 95% in the absence of oxygen. Amastigotes were relatively less affected by lack of oxygen and produced carbon dioxide at a rate comparable to promastigotes under anaerobic conditions. The presence of carbohydrates with a possible role in storage was detected in both promastigotes and amastigotes.
利用碳-13核磁共振技术，在有氧和无氧条件下测定了墨西哥利什曼原虫皮氏亚种无鞭毛体和前鞭毛体葡萄糖代谢的主要产物。在有氧条件下，两种形态的主要产物都是二氧化碳、琥珀酸、苹果酸、乙酸盐和丙氨酸。琥珀酸是前鞭毛体的主要代谢产物，而乙酸盐和丙氨酸在无鞭毛体中含量最为丰富。在无氧条件下，前鞭毛体产生甘油作为主要代谢产物，同时还有少量的琥珀酸、乙酸盐和丙氨酸；乙酸盐和丙氨酸仍然是无鞭毛体的主要代谢产物，琥珀酸的相对含量增加，并且产生了一些甘油。在有氧条件下，前鞭毛体产生二氧化碳的速率比无鞭毛体高5倍，但在无氧条件下，这一速率降低了95%以上。无鞭毛体受缺氧的影响相对较小，在无氧条件下产生二氧化碳的速率与前鞭毛体相当。在前鞭毛体和无鞭毛体中都检测到了可能具有储存作用的碳水化合物。
