肌动噬菌体RP3的DNA位点特异性整合到龟裂链霉菌假定的tRNA(Arg)(AGG)基因中。
The actinophage RP3 DNA integrates site-specifically into the putative tRNA(Arg)(AGG) gene of Streptomyces rimosus.
作者信息
Gabriel K, Schmid H, Schmidt U, Rausch H
机构信息
Institut für Genetik und Mikrobiologie, Universität München, Germany.
出版信息
Nucleic Acids Res. 1995 Jan 11;23(1):58-63. doi: 10.1093/nar/23.1.58.
Abstract
The temperate actinophage RP3 integrates site-specifically into the chromosome of Streptomyces rimosus R6-554. The phage attachment site attP and the hybrid attachment sites of the integrated prophage--attL and attR--were cloned and sequenced. The 54nt core sequence, common to all RP3 related attachment sites, comprises the 3' terminal end of a putative tRNA(Arg)(AGG) gene. AttB bears the complete tRNA gene which is restored in attL after integration. A 7.5kb HindIII fragment, bearing attP, was used to construct an integrative plasmid to simulate the integration process in vivo and to localize the phage genes necessary for site specific integration. The int and xis genes were sequenced and compared to other recombination genes.
摘要
温和型肌动噬菌体RP3特异性地整合到龟裂链霉菌R6 - 554的染色体中。噬菌体附着位点attP以及整合原噬菌体的杂合附着位点attL和attR被克隆并测序。所有与RP3相关的附着位点共有的54nt核心序列包含一个假定的tRNA(Arg)(AGG)基因的3'末端。AttB带有完整的tRNA基因，该基因在整合后在attL中得以恢复。携带attP的7.5kb HindIII片段用于构建整合质粒，以模拟体内的整合过程并定位位点特异性整合所需的噬菌体基因。对int和xis基因进行了测序，并与其他重组基因进行了比较。
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