肌动噬菌体RP3基因组粘性末端的序列分析及可转导穿梭载体的构建。
Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector.
作者信息
Kinner E, Pocta D, Ströer S, Schmieger H
机构信息
Institut für Genetik und Mikrobiologie, Universität München, FRG.
出版信息
FEMS Microbiol Lett. 1994 May 15;118(3):283-9. doi: 10.1111/j.1574-6968.1994.tb06842.x.
Abstract
The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.
摘要
肌动噬菌体RP3 DNA粘性末端的序列已被确定。与目前已研究的所有其他革兰氏阳性菌噬菌体一样，RP3 DNA具有3'突出末端。构建了一种穿梭粘粒，其中包含可被噬菌体RP3有效转导至龟裂链霉菌宿主细胞的粘性末端区域。
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