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肌动噬菌体RP3基因组粘性末端的序列分析及可转导穿梭载体的构建。

Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector.

作者信息

Kinner E, Pocta D, Ströer S, Schmieger H

机构信息

Institut für Genetik und Mikrobiologie, Universität München, FRG.

出版信息

FEMS Microbiol Lett. 1994 May 15;118(3):283-9. doi: 10.1111/j.1574-6968.1994.tb06842.x.

DOI:10.1111/j.1574-6968.1994.tb06842.x
PMID:7545934
Abstract

The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.

摘要

肌动噬菌体RP3 DNA粘性末端的序列已被确定。与目前已研究的所有其他革兰氏阳性菌噬菌体一样，RP3 DNA具有3'突出末端。构建了一种穿梭粘粒，其中包含可被噬菌体RP3有效转导至龟裂链霉菌宿主细胞的粘性末端区域。

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引用本文的文献

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Folia Microbiol (Praha). 2001;46(6):483-7. doi: 10.1007/BF02817990.
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The actinophage RP3 DNA integrates site-specifically into the putative tRNA(Arg)(AGG) gene of Streptomyces rimosus.肌动噬菌体RP3的DNA位点特异性整合到龟裂链霉菌假定的tRNA(Arg)(AGG)基因中。
Nucleic Acids Res. 1995 Jan 11;23(1):58-63. doi: 10.1093/nar/23.1.58.