采用聚合酶链反应-限制性片段长度多态性方法对日本寻常型天疱疮患者进行HLA-DQA1、-DQB1和-DRB1基因分型。
HLA-DQA1, -DQB1 and -DRB1 genotyping in Japanese pemphigus vulgaris patients by the PCR-RFLP method.
作者信息
Niizeki H, Inoko H, Mizuki N, Inamoto N, Watababe K, Hashimoto T, Nishikawa T
机构信息
Section of Dermatology, Kitasato Institute Hospital, Tokyo, Japan.
出版信息
Tissue Antigens. 1994 Oct;44(4):248-51. doi: 10.1111/j.1399-0039.1994.tb02390.x.
We performed HLA-DQA1, -DQB1 and -DRB1 genotyping using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for 32 Japanese pemphigus vulgaris (PV) patients. There was a significant association of either DQB10503 or DRB11405 with PV, and a negative association of either DQA10103 or DQB10601 with PV was found. Since the DQB1*0503+ patients had various DR14-related alleles, we concluded that the association with DQB1 is primary and that the association with DRB1 is simply due to linkage disequilibrium between the DQ and DR genes. These results may indicate that specific HLA class II antigens confer the susceptibility to PV among Japanese.
我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对32例日本寻常型天疱疮（PV）患者进行了HLA-DQA1、-DQB1和-DRB1基因分型。发现DQB10503或DRB11405与PV存在显著关联，同时发现DQA10103或DQB10601与PV存在负相关。由于DQB1*0503阳性患者具有多种与DR14相关的等位基因，我们得出结论，与DQB1的关联是主要的，而与DRB1的关联仅仅是由于DQ和DR基因之间的连锁不平衡。这些结果可能表明，特定的HLA-II类抗原赋予了日本人对PV的易感性。
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