Moreno M, Lark R, Campbell K L, Goodman J M
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
Yeast. 1994 Nov;10(11):1447-57. doi: 10.1002/yea.320101108.
Candida boidinii is a methylotrophic yeast in which several growth substrates can cause vigorous peroxisomal proliferation. While such diverse substrates as methanol, oleic acid and D-alanine induce different peroxisomal metabolic pathways, membranes seem to contain common abundant peroxisomal membrane proteins (PMPs). These proteins have been termed PMP31, PMP32 and PMP47. The gene encoding PMP47 has been previously cloned and analysed. We now report the isolation of a second PMP47 gene (or allele) as well as PMP31 and PMP32. PMP47A and PMP47B share 95% sequence identity at the amino acid level. PMP31 and PMP32 each contain 256 amino acids and are highly similar (97% identity) in protein sequence. Both PMP31 and PMP32 are predicted to span the membrane once or twice. All abundant PMPs of C. boidinii are basic in charge; they all have predicted isoelectric points above 10. RNAs corresponding to the PMP47s and to PMPs31-32 are strongly induced by methanol, oleic acid and D-alanine. While the PMP47s probably encode substrate carriers, the functions of PMP31 and PMP32 from C. boidinii are still unknown.
博伊丁假丝酵母是一种甲基营养型酵母，几种生长底物可导致其过氧化物酶体大量增殖。尽管甲醇、油酸和D - 丙氨酸等多种底物会诱导不同的过氧化物酶体代谢途径，但膜中似乎含有常见的丰富过氧化物酶体膜蛋白（PMPs）。这些蛋白被称为PMP31、PMP32和PMP47。编码PMP47的基因先前已被克隆和分析。我们现在报告第二个PMP47基因（或等位基因）以及PMP31和PMP32的分离。PMP47A和PMP47B在氨基酸水平上具有95%的序列同一性。PMP31和PMP32各含有256个氨基酸，蛋白质序列高度相似（97%同一性）。预计PMP31和PMP32都跨膜一次或两次。博伊丁假丝酵母所有丰富的PMPs都带正电荷；它们的预测等电点都高于10。与PMP47s以及PMP31 - 32相对应的RNA受到甲醇、油酸和D - 丙氨酸的强烈诱导。虽然PMP47s可能编码底物载体，但博伊丁假丝酵母中PMP31和PMP32的功能仍然未知。
