过氧化物酶体整合膜蛋白PMP47的分选序列包含在一个短的亲水环中。
The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop.
作者信息
Dyer J M, McNew J A, Goodman J M
机构信息
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041, USA.
出版信息
J Cell Biol. 1996 Apr;133(2):269-80. doi: 10.1083/jcb.133.2.269.
Abstract
No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening loop. We now demonstrate that the 20 amino acid loop, which is predicted to face the matrix, is both necessary and sufficient for peroxisomal targeting. Sufficiency was demonstrated with both chloramphenicol acetyltransferase and green fluorescent protein as carriers. There is a cluster of basic amino acids in the middle of the loop that we predict protrudes from the membrane surface into the matrix by a flanking stem structure. We show that the targeting signal is composed of this basic cluster and a block of amino acids immediately down-stream from it.
摘要
目前尚未确定过氧化物酶体整合膜蛋白的靶向序列。我们之前已经表明，一个67个氨基酸的区域对于将Pmp47（一种跨膜六次的蛋白质）靶向到过氧化物酶体是必需的。该区域包括两个膜跨段和中间的环。我们现在证明，预计面向基质的20个氨基酸的环对于过氧化物酶体靶向既是必需的也是充分的。以氯霉素乙酰转移酶和绿色荧光蛋白作为载体证明了其充分性。环的中间有一簇碱性氨基酸，我们预测它通过侧翼茎结构从膜表面突出到基质中。我们表明，靶向信号由这个碱性簇及其下游紧邻的一段氨基酸组成。
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