Grote J J, Hesseling S C, Tjebbes G J, van Blitterswijk C A
Department of Otorhinolaryngology, Leiden University Hospital, The Netherlands.
Ann Otol Rhinol Laryngol. 1995 Mar;104(3):226-30. doi: 10.1177/000348949510400308.
The effect of human monoclonal antibody HA-1A (Centoxin) on the effect of endotoxin on cultured rat middle ear epithelium was investigated. The addition of endotoxin to the standard culture medium revealed a concentration-related proliferative effect on cultured rat middle ear epithelium, leading to cobblestone cells, cell tracks, and stratification of epithelium, whereas rat middle ear epithelium cultured in standard medium grew as a monolayer composed of flat polygonal cells. Addition of HA-1A to standard medium supplemented with endotoxin gave rise to a statistically significant suppression of the proliferative effects of endotoxin on these cells. The morphology of rat middle ear epithelium cultured in the presence of HA-1A and endotoxin showed that these cells still had a tendency to form cobblestone-like cells and cell tracks, but to a substantially lower degree. The present results support the hypothesis that HA-1A suppresses the proliferative and morphological effects of endotoxin on rat middle ear epithelium and may play an important role in the pathogenesis of otitis media.
研究了人单克隆抗体HA-1A（Centoxin）对内毒素对培养的大鼠中耳上皮细胞作用的影响。在标准培养基中添加内毒素显示出对培养的大鼠中耳上皮细胞有浓度相关的增殖作用，导致鹅卵石样细胞、细胞轨迹和上皮分层，而在标准培养基中培养的大鼠中耳上皮细胞则生长为单层扁平多边形细胞。在添加了内毒素的标准培养基中加入HA-1A，可使内毒素对这些细胞的增殖作用受到统计学上显著的抑制。在HA-1A和内毒素存在的情况下培养的大鼠中耳上皮细胞的形态表明，这些细胞仍有形成鹅卵石样细胞和细胞轨迹的倾向，但程度要低得多。目前的结果支持这样的假说，即HA-1A可抑制内毒素对大鼠中耳上皮细胞的增殖和形态学作用，并可能在中耳炎的发病机制中起重要作用。
