Sedlak J, Duraj J, Hunakova L, Chorvath B
Cancer Research Institute, Bratislava, Slovakia.
Anticancer Res. 1994 Nov-Dec;14(6B):2473-6.
Monoclonal antibodies of the CD66/67 panel from the 5th Workshop on Leukocyte Antigens bound (as determined by flow cytometry) to the cell surface of human breast carcinoma cell lines BT-20 and MDA-MB-468. The molecular weight of antigenic polypeptides recognized by the CD66 monoclonal antibody F34-187 differed between the two examined breast carcinoma lines as follows: 50kDa, 95 kDa and 130 kDa polypeptides were expressed on both BT-20 and MCF-7 cell lines, while the major 160-180 kDa polypeptide was found only in MDA-MB-468 cells. IFN-gamma, all-trans retinoic acid and phorbol ester (TPA) induced up-regulation of CD66 antigen(s) recognized by the monoclonal antibody F34-187 (as determined by flow cytometry). Different alterations of CD66 antigenic polypeptides recognized by F34-187 monoclonal antibody, induced by interferon-gamma and indentified by immunoblotting, were found on BT-20, MCF-7 and MDA-MB-468 breast carcinoma cell lines.
来自白细胞抗原第5次研讨会的CD66/67组单克隆抗体（通过流式细胞术测定）与人类乳腺癌细胞系BT - 20和MDA - MB - 468的细胞表面结合。CD66单克隆抗体F34 - 187识别的抗原多肽的分子量在两个检测的乳腺癌细胞系之间有所不同，如下所示：50kDa、95kDa和130kDa的多肽在BT - 20和MCF - 7细胞系中均有表达，而主要的160 - 180kDa多肽仅在MDA - MB - 468细胞中发现。γ干扰素、全反式维甲酸和佛波酯（TPA）诱导了单克隆抗体F34 - 187识别的CD66抗原上调（通过流式细胞术测定）。在BT - 20、MCF - 7和MDA - MB - 468乳腺癌细胞系中发现，由γ干扰素诱导并通过免疫印迹鉴定的F34 - 187单克隆抗体识别的CD66抗原多肽存在不同改变。
