Chang C F, Lin S J
Department of Aquaculture, National Taiwan Ocean University, Keelung, Republic of China.
Reprod Nutr Dev. 1995;35(1):95-103. doi: 10.1051/rnd:19950107.
The objective of this study was to investigate the effects of bovine insulin on growth responses in tilapia. Juvenile hybrid male tilapia (Oreochromis niloticus x O aurea; n = 135) were subjected to 1 of 3 treatments. Each treatment was subdivided into 3 replicates of 15 fish each. The fish were immersed into 1 of 2 doses (10 and 100 micrograms/100 ml water) of insulin or no hormone for 15 min per week for 8 weeks. Body weight, growth and feed conversion efficiency were significantly higher as a result of the first 4 weeks of insulin treatment as compared to control fish. The lower dose of insulin had a better stimulation effect than that of the higher doses. Insulin also stimulated feed consumption. Liver protein and protein/DNA ratio were higher in both insulin-treated groups than in the control group. Muscle proximate composition and hepatosomatic index were similar in the insulin-treated and control groups. The experimental findings suggest that insulin administered by immersion can enhance growth, feed consumption, food utilization and liver cell size in tilapia.
本研究的目的是调查牛胰岛素对罗非鱼生长反应的影响。将杂交雄性幼罗非鱼（尼罗罗非鱼×奥利亚罗非鱼；n = 135）进行三种处理中的一种。每种处理再细分为3个重复组，每组15条鱼。每周将鱼浸入两种剂量（10和100微克/100毫升水）的胰岛素或无激素溶液中15分钟，持续8周。与对照鱼相比，胰岛素处理的前4周后，体重、生长和饲料转化效率显著更高。较低剂量的胰岛素比较高剂量具有更好的刺激效果。胰岛素还刺激了饲料消耗。两个胰岛素处理组的肝脏蛋白质和蛋白质/DNA比值均高于对照组。胰岛素处理组和对照组的肌肉近似成分和肝体指数相似。实验结果表明，通过浸泡给予胰岛素可提高罗非鱼的生长、饲料消耗、食物利用率和肝细胞大小。
