Effects of dietary supplementation with green tea waste on growth, digestive enzyme and lipid metabolism of juvenile hybrid tilapia, Oreochromis niloticus × O. aureus.

An 8-week feeding trial was conducted to evaluate the effects of dietary supplementation with green tea waste (GTW) on growth, digestive enzyme and lipid metabolism of juvenile hybrid tilapia, Oreochromis niloticus × O. aureus. The fish (initial mean body weight, 12.63 ± 0.75 g) were fed five experimental diets that included 0 (control), 0.8, 1.6, 3.2 or 6.4 % of GTW in triplicate aquaria, twice daily. Growth performance, plasma metabolites content and liver and intestine digestive enzyme activities were determined. Fish accepted well all experimental diets during the trial, and no mortality was observed. The weight gain increased (P < 0.05) with the increase in GTW inclusion level up to 1.6 %, after which it decreased, but no significant differences between the control and high level (3.2 or 6.4 % of GTW) groups were observed. Moreover, fish fed on diets containing 0.8 and 1.6 % GTW had lower feed conversion ratio (FCR, 1.75 and 1.73, respectively) and had better protein deposition (higher protein efficiency ratio, PER, 1.73 and 1.71, respectively), compared to other treatments. No differences among groups were observed in whole body and dorsal muscle composition with the exception of lipid content which was lower in fish fed 6.4 % GTW diets, compared to other treatments. Lipase activities in liver or intestine were higher in fish fed GTW-supplemented diets with the exception of intestine lipase activities, which was unaffected, compared to the control. Similarly, liver lipoprotein lipase activities were also increased in fish fed diets supplemented a medium dose of GTW (1.6 or 3.2 %), compared to other treatments. However, intestine amylase activities were decreased in fish fed diets containing a high dose of GTW (3.2 and 6.4 %); while the liver amylase activities were unaffected by the GTW supplementation. Blood chemistry parameters were affected by GTW inclusion, except the values of triglycerides, which was unaffected. The values of total cholesterol, HDL cholesterol and LDL cholesterol increased with increasing GTW inclusion level up to 3.2 %, after which the values decreased. These results indicate that diets supplemented with appropriate concentration of GTW (from 0.8 to 1.6 %) may potentially serve as an effective functional food and additive for tilapia to improve growth performance, digestion efficacy and fat metabolism.