Akagawa K
Department of Immunology, National Institute of Health Science, Tokyo, Japan.
Hum Cell. 1994 Sep;7(3):116-20.
We found that human monocytes differentiate into macrophages (Mp) by GM-CSF and M-CSF. The Mp induced by GM-CSF and M-CSF are different in their morphology, cell surface antigen expression and functions. In the course of that study, we found that IL-4 modulate the differentiation of monocytes induced by GM-CSF and M-CSF. IL-4 alone did not induce the proliferation and differentiation of monocytes. IL-4, however, inhibited the proliferative response of monocytes to GM-CSF. When monocytes were incubated with GM-CSF and IL-4 simultaneously, the cells recovered were non-adherent, non-phagocytic, and did not form rosette with EA. The cells were also negative in nonspecific esterase and showed an appearance of dendritic cells (DC). The DC-like cells expressed CD1, DR, DQ and CD11c, but not CD14, CD71 and 710F. The cells showed strong APC activity in alogeneic and autologous mixed lymphocyte reaction (MLR). When monocytes were incubated with M-CSF and IL-4, TRAP positive multinucleated giant cells appeared. Taken together, these results suggest that IL-4 is a principal factor that control the differential development of human monocytes into DC and multinucleated giant cells.
我们发现人类单核细胞可通过粒细胞-巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞集落刺激因子（M-CSF）分化为巨噬细胞（Mp）。GM-CSF和M-CSF诱导产生的Mp在形态、细胞表面抗原表达及功能方面存在差异。在该项研究过程中，我们发现白细胞介素-4（IL-4）可调节GM-CSF和M-CSF诱导的单核细胞分化。单独的IL-4不会诱导单核细胞的增殖和分化。然而，IL-4会抑制单核细胞对GM-CSF的增殖反应。当单核细胞同时与GM-CSF和IL-4孵育时，回收的细胞不黏附、不吞噬，也不与致敏红细胞（EA）形成玫瑰花结。这些细胞的非特异性酯酶也呈阴性，并呈现出树突状细胞（DC）的形态。类DC细胞表达CD1、DR、DQ和CD11c，但不表达CD14、CD71和710F。这些细胞在同种异体和自体混合淋巴细胞反应（MLR）中表现出较强的抗原呈递细胞（APC）活性。当单核细胞与M-CSF和IL-4孵育时，会出现抗酒石酸酸性磷酸酶（TRAP）阳性的多核巨细胞。综上所述，这些结果表明IL-4是控制人类单核细胞向DC和多核巨细胞分化发育的主要因子。
