[抗变性DNA抗体对人骨髓细胞在半固体琼脂中形成集落的影响]
[Effect of antibodies to denatured DNA on the human bone marrow cells forming colonies in semi-solid agar].
作者信息
Kolesnikova A I, Pedgorodnichenko V K, Poverennyĭ A M, Zherbin E A
出版信息
Biull Eksp Biol Med. 1978 Mar;85(3):318-20.
gamma-globulin from rabbit sera containing antibodies to denatured DNA or cytidine suppressed the efficiency of colony formation in agar cultures of human bone marrow cells. The antibodies to DNA isolated from the immune sera by the immunosorbent produced an analogous effect. gamma-globulin from the sera of intact animals and immune sera after the removal of antibodies to denatured DNA from them produced a different effect. gamma-globulin in a concentration of 0.28 and 5 mg failed to influence the colony-formation of 2.10(5) explanted nucleus-containing cells, by stimulated the colony growth when added in a concentration of 15 mg.
含有抗变性DNA或胞嘧啶抗体的兔血清γ球蛋白抑制了人骨髓细胞琼脂培养中的集落形成效率。通过免疫吸附从免疫血清中分离出的抗DNA抗体产生了类似的效果。来自完整动物血清的γ球蛋白以及去除其中抗变性DNA抗体后的免疫血清产生了不同的效果。浓度为0.28毫克和5毫克的γ球蛋白未能影响2×10⁵个接种的含细胞核细胞的集落形成，但当以15毫克的浓度添加时会刺激集落生长。
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