Strotkamp D, Roos P H, Hanstein W G
Institute for Physiological Chemistry, Ruhr University Bochum, Germany.
Biochim Biophys Acta. 1995 Feb 21;1260(3):341-4. doi: 10.1016/0167-4781(94)00244-w.
A cDNA library constructed from adult female Sprague Dawley rat liver was screened with polyclonal anti CYP3A-IgG. One of the positive clones, cUT, was found to contain the complete coding sequence of a new gene more similar to hamster gene CYP3A10 (cDNA: 85%, deduced amino acid sequence: 79%) than to the known rat CYP3A genes (cDNA: 75-76%, amino acid sequences: 66-69%). The deduced sequence of the first 28 amino acids is identical to the N-terminus of the testosterone 6 beta-hydroxylase, 6 beta-2 (Nagata, K., Gonzalez, F.J., Yamazoe, Y. and Kato, R. (1990) J. Biochem. 107, 718-725). Northern blots show the presence of cUT mRNA in livers of adult female and male rats. The transcription of the new gene is enhanced in either sex by pregnenolone-alpha-carbonitrile, dexamethasone, phenobarbital, and triacetyloleandomycin, known inducers of CYP3A gene expression.
用多克隆抗CYP3A-IgG筛选从成年雌性斯普拉格-道利大鼠肝脏构建的cDNA文库。发现其中一个阳性克隆cUT含有一个新基因的完整编码序列，该基因与仓鼠基因CYP3A10（cDNA：85%，推导氨基酸序列：79%）的相似性高于已知的大鼠CYP3A基因（cDNA：75 - 76%，氨基酸序列：66 - 69%）。推导的前28个氨基酸序列与睾酮6β-羟化酶6β-2的N端相同（永田，K.，冈萨雷斯，F.J.，山添，Y.和加藤，R.（1990年）《生物化学杂志》107，718 - 725）。Northern印迹显示成年雌性和雄性大鼠肝脏中存在cUT mRNA。该新基因的转录在任一性别中都可被孕烯醇酮-α-腈、地塞米松、苯巴比妥和三乙酰竹桃霉素增强，这些都是已知的CYP3A基因表达诱导剂。
