Senkovich O A, Chernitsky E A
Institute of Photobiology, Academy of Sciences of the Republic of Belarus, Minsk, Republic of Belarus. volot%
Membr Cell Biol. 1998;11(5):679-89.
The size of pores arising in human erythrocytes under the action of two detergents (Triton X-100 and sodium dodecyl sulfate) and causing the slow component of hemolysis was estimated by the method of osmotic protectors. The pore diameters were found to be about 40 A. The pores responsible for the fast component of hemolysis induced by sodium dodecyl sulfate were shown to be of greater size and even molecules of polyethylene glycol 4000 could pass through them. The unusual decrease. In the rate and percentage of this hemolytic component was caused by the side action of the protectors, i.e., by the acceleration of erythrocyte vesiculation, a process that competed with pore formation. Vesiculation protected erythrocytes against fast and slow detergent-induced hemolysis. It is shown that the method of osmotic protectors can not be used for estimation of pore size in fast hemolysis by sodium dodecyl sulfate. The application of this method for hemolysis by other amphiphilic compounds is discussed.
采用渗透保护剂法估算了两种去污剂（曲拉通X-100和十二烷基硫酸钠）作用于人体红细胞时产生的、导致溶血慢成分的孔的大小。发现孔径约为40埃。结果表明，由十二烷基硫酸钠诱导的溶血快成分所对应的孔更大，甚至聚乙二醇4000分子都能通过。这种溶血成分的速率和百分比出现异常降低是由保护剂的副作用引起的，即由红细胞囊泡化加速所致，这一过程与孔的形成相互竞争。囊泡化保护红细胞免受去污剂诱导的快速和慢速溶血。结果表明，渗透保护剂法不能用于估算十二烷基硫酸钠快速溶血时的孔径。讨论了该方法在其他两亲性化合物溶血中的应用。
