Guzaev A, Azhayeva E, Hovinen J, Azhayev A, Lönnberg H
Department of Chemistry, University of Turku, Finland.
Bioconjug Chem. 1994 Nov-Dec;5(6):501-3. doi: 10.1021/bc00030a003.
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.
已制备了标准M13测序引物的几种类似物，这些类似物含有多达五个3'-脱氧胸苷，或在1'-位置用荧光素标记的一个或两个这样的单元。所有这些寡核苷酸均已证明能引发DNA聚合酶催化的DNA合成。
