Umeno Daisuke, Kimura Yuki, Kawai-Noma Shigeko
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan.
Anal Sci. 2021 May 10;37(5):699-703. doi: 10.2116/analsci.20SCR12. Epub 2021 Jan 22.
One of the most prominent features of genetically encoded biosensors (GEBs) is their evolvability-the ability to invent new sensory functions using mutations. Among the GEBs, the transcription factor-based biosensors (TF-biosensors) is the focus of this review. We also discuss how this class of sensors can be highly evolvable and how we can exploit it. With an established platform for directed evolution, researchers can create, or evolve, new TF-biosensors. Directed evolution experiments have revealed the TF-biosensors' evolvability, which is based partially on their characteristic physicochemical properties.
基因编码生物传感器（GEB）最显著的特征之一是其可进化性——即利用突变创造新传感功能的能力。在基因编码生物传感器中，基于转录因子的生物传感器（TF生物传感器）是本综述的重点。我们还将讨论这类传感器如何具有高度可进化性以及如何加以利用。借助成熟的定向进化平台，研究人员能够创建或进化出新的TF生物传感器。定向进化实验揭示了TF生物传感器的可进化性，这部分基于其独特的物理化学性质。
