Peterka M, Sarin S, Roeder E, Wiedenfeld H, Halasková M
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Praha.
Funct Dev Morphol. 1994;4(2):89-92.
The embryotoxic properties of pyrrolizidine alkaloids senecionine (water insoluble) and senecionine-N-oxide (water soluble) were examined using the CHEST method (Chick Embryotoxicity Screening Test). The beginning of the embryotoxicity range for senecionine was found to be between 3-30 micrograms; senecionine-N-oxide, on the other hand, showed no effect even with 100 micrograms dose. On the basis of the theoretical calculation, it can be expected that senecionine embryotoxicity for mammals ranges between 10-100 mg/kg maternal body weight, overlapping in this way the known adult toxicity range. The rat LD50 of most alkaloids known to be significant for human health are in the range of 34-300 mg/kg.
使用CHEST方法（鸡胚毒性筛选试验）检测了吡咯里西啶生物碱千里光碱（水不溶性）和千里光碱-N-氧化物（水溶性）的胚胎毒性特性。发现千里光碱的胚胎毒性范围起始于3至30微克之间；另一方面，即使剂量为100微克，千里光碱-N-氧化物也未显示出任何影响。根据理论计算，可以预期千里光碱对哺乳动物的胚胎毒性范围在母体体重10至100毫克/千克之间，以此方式与已知的成人毒性范围重叠。已知对人类健康有重要影响的大多数生物碱的大鼠半数致死量在34至300毫克/千克范围内。
