来自C3H/HeJ和C3H/OuJ小鼠的巨噬细胞激活所需的不同信号要求。

Differing signal requirements for the activation of macrophages from C3H/HeJ and C3H/OuJ mice.

作者信息

Abu-Lawi K I, Sultzer B M

机构信息

Department of Microbiology and Immunology, State University of New York, Health Science Center at Brooklyn 11203.

出版信息

FEMS Immunol Med Microbiol. 1994 Nov;10(1):31-8. doi: 10.1111/j.1574-695X.1994.tb00008.x.

DOI:10.1111/j.1574-695X.1994.tb00008.x

PMID:7874076

Abstract

Endotoxin-associated protein (EP) from Salmonella typhi stimulated the release of prostaglandin E2 (PGE2), interleukin-1 (IL-1), and interferon (IFN) activity in macrophages from the lipopolysaccharide (LPS) responder C3H/OuJ mouse strain. However, only PGE2 and IL-1 were stimulated by EP in macrophages from the LPS nonresponder C3H/HeJ mouse strain. LPS stimulated the release of PGE2, IL-1 and IFN activity in C3H/OuJ macrophages, but not in C3H/HeJ macrophages. The protein kinase C (PKC) activator phorbol myristic acid (PMA) stimulated PGE2 production in both strains but not IL-1 production, suggesting that signalling pathways other than PKC may be involved in IL-1 production. The calcium ionophore ionomycin stimulated PGE2 production in C3H/OuJ but not C3H/HeJ macrophages, suggesting a defective calcium-related pathway in the C3H/HeJ macrophages as compared to the C3H/OuJ cells.

摘要

伤寒沙门氏菌的内毒素相关蛋白（EP）刺激了脂多糖（LPS）反应型C3H/OuJ小鼠品系巨噬细胞中前列腺素E2（PGE2）、白细胞介素-1（IL-1）和干扰素（IFN）活性的释放。然而，在LPS无反应型C3H/HeJ小鼠品系的巨噬细胞中，EP仅刺激了PGE2和IL-1的释放。LPS刺激了C3H/OuJ巨噬细胞中PGE2、IL-1和IFN活性的释放，但未刺激C3H/HeJ巨噬细胞。蛋白激酶C（PKC）激活剂佛波醇肉豆蔻酸酯（PMA）刺激了两个品系中PGE2的产生，但未刺激IL-1的产生，这表明除PKC外的其他信号通路可能参与IL-1的产生。钙离子载体离子霉素刺激了C3H/OuJ巨噬细胞中PGE2的产生，但未刺激C3H/HeJ巨噬细胞，这表明与C3H/OuJ细胞相比，C3H/HeJ巨噬细胞中与钙相关的信号通路存在缺陷。