Flebbe L M, Chapes S K, Morrison D C
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
J Immunol. 1990 Sep 1;145(5):1505-11.
We have investigated the relative immunostimulatory activities of S-chemotype LPS and R-chemotype LPS preparations on C3H/HeJ peritoneal macrophages in vitro. As assessed by either secretion of TNF-alpha or IL-1, some of the R-chemotype LPS manifest significant activity on these normally LPS-unresponsive cells. The expression of IL-1 activity by R-LPS-stimulated C3H/HeJ macrophages was unaffected by IFN-gamma; however, this cytokine significantly enhanced TNF-alpha production by the same cells. The R-chemotype LPS preparations alone were not able to activate C3H/HeJ macrophages to become tumoricidal but activity could readily be demonstrated in the presence of IFN-gamma. Of potential importance is the observation that the profile of relative activity of the various R-chemotype LPS preparations for macrophage activation does not parallel that previously obtained by us for the C3H/HeJ B-lymphocyte activation.
我们已经在体外研究了S型脂多糖（LPS）和R型脂多糖制剂对C3H/HeJ腹膜巨噬细胞的相对免疫刺激活性。通过肿瘤坏死因子-α（TNF-α）或白细胞介素-1（IL-1）的分泌评估，一些R型脂多糖对这些通常对LPS无反应的细胞表现出显著活性。R-LPS刺激的C3H/HeJ巨噬细胞中IL-1活性的表达不受γ干扰素（IFN-γ）的影响；然而，这种细胞因子显著增强了相同细胞中TNF-α的产生。单独的R型脂多糖制剂不能激活C3H/HeJ巨噬细胞使其具有杀肿瘤活性，但在IFN-γ存在下活性很容易得到证明。具有潜在重要性的是观察到，各种R型脂多糖制剂对巨噬细胞激活的相对活性谱与我们之前获得的C3H/HeJ B淋巴细胞激活的谱不平行。
