一种针对互补肽的单克隆自身抗体可识别间质胶原酶。
A monoclonal autoantibody against a complementary peptide recognizes interstitial collagenase.
作者信息
de Souza S J, Madaio M P, Juliano Neto L, Brentani R R
机构信息
Ludwig Institute for Cancer Research, São Paulo Branch, Brazil.
出版信息
Immunomethods. 1994 Oct;5(2):172-6. doi: 10.1006/immu.1994.1052.
A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernantant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.
本文描述了一种针对互补肽TKKTLRT的单克隆自身抗体（mab 16），该互补肽是从胶原蛋白中对胶原酶敏感的位点推导出来的。通过ELISA和免疫沉淀试验可见，mab 16能识别间质胶原酶。此外，mab 16能够部分抑制角质形成细胞上清液的胶原分解活性。最后，使用mab 16/琼脂糖柱对胶原酶进行免疫纯化成为可能。
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