Maluchnik D, Borsuk E
Department of Embryology, University of Warsaw, Poland.
Zygote. 1994 May;2(2):129-31. doi: 10.1017/s096719940000188x.
We have investigated the oolemma block to polyspermy in the mouse. Zona-free and zona-intact eggs were fertilised and subsequently re-inseminated (the latter following zona pellucida removal). The 'perivitelline' block to polyspermy in zona-intact eggs renders motile sperm in the perivitelline space unable to bind to the oolemma. This is not connected with irreversible changes in the egg plasma membrane, because freshly added sperm can still fuse with such eggs freed from the zona. Fertilised eggs eventually lose the ability to fuse with sperm within 1 h, while still being able to bind many sperm.
我们研究了小鼠卵膜对多精受精的阻断作用。去除透明带和保留透明带的卵母细胞均进行受精，随后再次授精（后者在去除透明带后进行）。保留透明带的卵母细胞对多精受精的“卵周”阻断作用使卵周隙中的活动精子无法与卵膜结合。这与卵质膜的不可逆变化无关，因为新加入的精子仍能与去除透明带的此类卵母细胞融合。受精后的卵母细胞最终在1小时内失去与精子融合的能力，但其仍能结合许多精子。
