O'Hare D B, Robotham P W, Gill R
School of Environmental and Applied Sciences, Department of Biology, University of Derby, U.K.
Chemosphere. 1995 Jan;30(2):257-64. doi: 10.1016/0045-6535(94)00388-b.
The use of hepatic post-mitochondrial supernatant (pms) as a source of monooxygenase activity in roach following intra peritoneal injection of beta-naphthoflavone was investigated. Pms was found to be three times less active than microsomes although the level of induction was similar (9 fold). No effect of dicumarol on Ethoxyresorufin O-deethylase (EROD) measurement using pms was recorded. EROD induction in roach peaked 2-4 days post treatment with beta-naphthoflavone. Given the ubiquitous nature and pollution tolerance of roach, their hepatic pms may serve as a convenient biomonitor of PAH contamination in the freshwater environment. Field studies are now underway to investigate this further.
研究了在腹腔注射β-萘黄酮后，将肝脏线粒体后上清液（PMS）用作蟑螂单加氧酶活性来源的情况。尽管诱导水平相似（9倍），但发现PMS的活性比微粒体低三倍。未记录双香豆素对使用PMS进行乙氧芴香豆素O-脱乙基酶（EROD）测量的影响。用β-萘黄酮处理后，蟑螂中的EROD诱导在2-4天达到峰值。鉴于蟑螂的广泛分布特性和对污染的耐受性，其肝脏PMS可作为淡水环境中多环芳烃污染的便捷生物监测指标。目前正在进行实地研究以进一步调查此事。
