Burgeot T, Bocquené G, Pingray G, Godefroy D, Legrand J, Dimeet J, Marco F, Vincent F, Henocque Y, Jeanneret H O
IFREMER, Rue de l'Ile d'Yeu, Nantes, France.
Ecotoxicol Environ Saf. 1994 Nov;29(2):131-47. doi: 10.1016/0147-6513(94)90015-9.
The use of bioindicators to evaluate exposure to the biological effects of chemical pollutants in marine organisms constitutes a new tool in the monitoring field. The establishment of a North Sea monitoring network in 1991, involving such international organizations as the North Sea Task Force, the International Council for the Exploration of the Sea, and the Intergovernmental Oceanography Commission, led French researchers to develop an enzymatic biomarker to monitor biological effects within the National Observation Network. The biomarker, ethoxyresorufin-O-deethylase (EROD), dependent on the CP450 system, has been monitored biannually since 1992 in several species of fish (Callionymus lyra, Limanda limanda, Serranus sp., Mullus barbatus) in two coastal sites particularly exposed to industrial and domestic pollution. A rapid method is used to assay EROD enzymatic activity determined along a pollution gradient, and results are interpreted on a microplate reader. The strategy of this approach is to assess the effects on the marine ecosystem during prolonged exposure to specific pollutants such as polyaromatic hydrocarbons, polychlorinated biphenyls, and dioxins.
利用生物指示物评估海洋生物接触化学污染物生物效应的情况，构成了监测领域的一种新工具。1991年建立北海监测网络，该网络涉及北海特别行动组、国际海洋考察理事会和政府间海洋学委员会等国际组织，这促使法国研究人员开发一种酶生物标志物，以在国家观测网络内监测生物效应。自1992年以来，这种依赖细胞色素P450系统的生物标志物——乙氧基异吩恶唑酮 - O - 脱乙基酶（EROD），在两个特别易受工业和生活污染影响的沿海地点，对几种鱼类（琴角鱼、黄盖鲽、锯鳃石鲈、条尾绯鲤）每半年监测一次。采用一种快速方法测定沿污染梯度的EROD酶活性，并在酶标仪上解读结果。这种方法的策略是评估长期接触多环芳烃、多氯联苯和二恶英等特定污染物对海洋生态系统的影响。
