Lemarre P, Robineau S, Colson A M, Netter P
Laboratoire de Génétique Microbienne, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Curr Genet. 1994 Nov-Dec;26(5-6):546-52. doi: 10.1007/BF00309948.
Three respiratory-deficient mutants of cytochrome oxidase subunit I in the yeast mitochondrion have been sequenced. They are located in, or near, transmembrane segment VI, the catalytic core of the enzyme. Respiratory-competent revertants have been selected and studied. The mutant V244M was found to revert at the same site in valine (wild-type), isoleucine or threonine. The revertants of the mutant G251R were of three types: glycine (wild-type), serine and threonine at position 251. A search for second-site mutations was carried out but none were found. Among 60 revertants tested, the mutant K265M was found to revert only to the wild-type allele.
已对酵母线粒体中细胞色素氧化酶亚基I的三个呼吸缺陷型突变体进行了测序。它们位于酶的催化核心跨膜片段VI内或附近。已筛选并研究了具有呼吸能力的回复突变体。发现突变体V244M在缬氨酸（野生型）、异亮氨酸或苏氨酸的同一位点回复突变。突变体G251R的回复突变体有三种类型：251位的甘氨酸（野生型）、丝氨酸和苏氨酸。进行了第二位点突变的搜索，但未发现任何突变。在测试的60个回复突变体中，发现突变体K265M仅回复为野生型等位基因。
