Meunier B, Colson A M
Universite Catholique de Louvain, Laboratoire de Genetique Microbienne, Louvain-la-Neuve, Belgium.
FEBS Lett. 1993 Dec 13;335(3):338-40. doi: 10.1016/0014-5793(93)80414-p.
In yeast, revertants were selected from two respiratory deficient mutants carrying mutations in the catalytic subunits of cytochrome oxidase. From a mutant carrying a double mutation in the vicinity of the copper binding pocket in subunit II, two genetically independent revertants were obtained in which the same extragenic reversion mutation was observed, A147V, in the putative helix 4 of subunit I. A comparison with revertants derived from the second deficient mutant, carrying the deficiency mutation, S140L, in the loop 3-4 of subunit I, provides additional data in favour of an interaction between helix 4 of subunit I and subunit II.
在酵母中，从两个细胞色素氧化酶催化亚基发生突变的呼吸缺陷型突变体中筛选回复突变体。从亚基II中铜结合口袋附近携带双突变的一个突变体中，获得了两个遗传上独立的回复突变体，在亚基I的假定螺旋4中观察到相同的基因外回复突变，A147V。与来自第二个缺陷型突变体的回复突变体进行比较，该突变体在亚基I的环3-4中携带缺陷突变S140L，这提供了更多数据支持亚基I的螺旋4与亚基II之间存在相互作用。
