Wang Z Y, Liu T H, Cui Q C
Department of Pathology, PUMC Hospital, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1994 Oct;23(5):270-3.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze c-Ki-ras codon 12 mutation in 27 fine needle aspiration biopsy (FNAB) specimens of the pancreas and its adjacent organs for the diagnosis of pancreatic adenocarcinoma. C-Ki-ras codon 12 mutation was present in 14 out of 15 cases of pancreatic adenocarcinoma, the positive rate was 93.33% (14/15); whereas no mutation was detected in those obtained from 12 patients with chronic pancreatitis, pancreatic cyst, gallbladder carcinoma, carcinoma of ampulla of Vater and gastric lymphoma. The results of this study verifies the PCR-RFLP technique as a practical, sensitive, rapid and reliable method for the detection of c-Ki-ras codon 12 mutation in the diagnosis of pancreatic adenocarcinoma.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对27例胰腺及其毗邻器官细针穿刺活检（FNAB）标本进行c-Ki-ras第12密码子突变分析，以诊断胰腺腺癌。15例胰腺腺癌中有14例存在c-Ki-ras第12密码子突变，阳性率为93.33%（14/15）；而12例慢性胰腺炎、胰腺囊肿、胆囊癌、壶腹癌和胃淋巴瘤患者的标本未检测到突变。本研究结果证实，PCR-RFLP技术是检测c-Ki-ras第12密码子突变以诊断胰腺腺癌的一种实用、灵敏、快速且可靠的方法。
