改进的聚合酶链反应扩增/ Hhal酶切用于明确载脂蛋白E等位基因的测定
Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.
作者信息
Appel E, Eisenberg S, Roitelman J
机构信息
Institute of Lipid and Atherosclerosis Research, Sheba Medical Center, Tel Hashomer, Israel.
出版信息
Clin Chem. 1995 Feb;41(2):187-90.
PMID:7874769
Abstract
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.
摘要
我们提出了一种针对人类载脂蛋白（apo）E的聚合酶链反应扩增/Hhal限制性分型方法的改进。该方法包括一个诱变正向引物和对两个引物的5'端进行标记。对Hixon和Vernier（《脂质研究杂志》1990年；31:545 - 8）所描述的原始方法的这些改进，能够灵敏且明确地确定apoE基因型。
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