Livesey J F, Perkins S L, Tokessy N E, Maddock M J
Department of Laboratory Medicine, Ottawa Civic Hospital, Ontario, Canada.
Clin Chem. 1995 Feb;41(2):300-5.
We developed a packed-column chromatographic procedure capable of simultaneous quantitation of methanol, ethanol, isopropanol, acetone, and ethylene glycol. This method was then updated to a rapid, sensitive, wide-bore capillary method. The packed-column system uses direct injection of 1 microL of Na2WO4/H2SO4-deproteinized serum onto a 1.8 m x 2 mm (i.d.) column packed with 80/100 HayeSep R. A linear temperature gradient from 90 to 205 degrees C allows complete elution of all components within 20 min; minimum detection limits are 2 mmol/L. The wide-bore capillary method uses 0.1 microL of sample deproteinized by ultrafiltration, injected onto a 30 m x 0.53 mm (i.d.) 3-microns Rtx-200 (Restek) column. Baseline resolution to a minimum detection limit of 0.1 mmol/L of all compounds is achieved in 5 min with a linear temperature gradient from 40 to 250 degrees C and dual internal standards of n-propanol and 1,2-butanediol.
我们开发了一种填充柱色谱程序，能够同时定量测定甲醇、乙醇、异丙醇、丙酮和乙二醇。该方法随后更新为一种快速、灵敏的宽口径毛细管方法。填充柱系统是将1微升经钨酸钠/硫酸脱蛋白的血清直接进样到一根1.8米×2毫米（内径）、填充有80/100 HayeSep R的色谱柱上。90至205摄氏度的线性温度梯度可在20分钟内实现所有组分的完全洗脱；最低检测限为2毫摩尔/升。宽口径毛细管方法使用0.1微升经超滤脱蛋白的样品，进样到一根30米×0.53毫米（内径）、3微米的Rtx - 200（Restek）色谱柱上。通过40至250摄氏度的线性温度梯度和正丙醇与1,2 - 丁二醇的双内标，在5分钟内可实现所有化合物的基线分离，最低检测限为0.1毫摩尔/升。
