Daniell T, Edwards R
Department of Biological Sciences, University of Durham, UK.
FEBS Lett. 1995 Feb 20;360(1):57-61. doi: 10.1016/0014-5793(95)00075-k.
The methylation of endogenous proteins increased in alfalfa cell suspension cultures following treatment with a fungal elicitor. Carboxyl methylation, a post-translational modification associated with controlling the localisation and longevity of proteins, was the dominant form of protein methylation in both elicited and unelicited cells. Protein methylation was restricted to a limited number of peptides prior to elicitor treatment but as elicitation progressed the number of endogenous substrates increased. Increases resulted from a combination of an elicitor-dependent increase in the activity of a protein carboxyl methyltransferase and the accumulation of preferred endogenous substrates in the latter stages of elicitation.
在用真菌激发子处理后，苜蓿细胞悬浮培养物中内源蛋白的甲基化增加。羧基甲基化是一种与控制蛋白质定位和寿命相关的翻译后修饰，是诱导和未诱导细胞中蛋白质甲基化的主要形式。在激发子处理之前，蛋白质甲基化仅限于有限数量的肽段，但随着激发过程的进行，内源性底物的数量增加。这种增加是由蛋白质羧基甲基转移酶活性的激发子依赖性增加和激发后期优选内源性底物的积累共同导致的。
