Krell T, Pitt A R, Coggins J R
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK.
FEBS Lett. 1995 Feb 20;360(1):93-6. doi: 10.1016/0014-5793(95)00083-l.
The arginine-specific reagent phenylglyoxal has been used to identify a hyper-reactive arginine residue which is essential for activity in the type II dehydroquinases of Streptomyces coelicolor and Aspergillus nidulans. Electrospray mass spectrometry was used both to characterise the phenylglyoxal modified protein, and to identify the phenylglyoxal modified peptides following enzymatic digestion. The advantages of using electrospray mass spectrometry for monitoring arginine modication aimed at identifying functional residues in proteins are discussed.
精氨酸特异性试剂苯乙二醛已被用于鉴定一个超反应性精氨酸残基，该残基对于天蓝色链霉菌和构巢曲霉的II型脱氢奎尼酸酶的活性至关重要。电喷雾质谱法既用于表征苯乙二醛修饰的蛋白质，也用于鉴定酶消化后苯乙二醛修饰的肽段。讨论了使用电喷雾质谱法监测旨在鉴定蛋白质中功能残基的精氨酸修饰的优点。
