Klare I, Heier H, Claus H, Reissbrodt R, Witte W
Robert Koch Institute, Wernigerode, Germany.
FEMS Microbiol Lett. 1995 Jan 15;125(2-3):165-71. doi: 10.1111/j.1574-6968.1995.tb07353.x.
Glycopeptide-resistant Enterococcus faecium strains were isolated from a pig farm and a poultry farm both using avoparcin as a food additive. Such organisms were not isolated in a hen's eggs-producing farm not using avoparcin. Glycopeptide-resistant enterococci were also detected in broiler chicken carcasses that were delivered to a hospital's kitchen. The resistance was determined by the vanA gene as indicated by the detection of the inducible 39-kDa cytoplasmic membrane protein and of a vanA-specific DNA sequence amplified by polymerase chain reaction. Genomic DNA fragment patterns of strains from animal sources were different from each other and also from those of strains isolated in hospitals and from sewage treatment plants. This findings suggest the dissemination of the vanA determinant among different enterococcal strains of distinct ecological origin.
从一个使用阿伏帕星作为食品添加剂的养猪场和一个养禽场中分离出了耐糖肽粪肠球菌菌株。在一个不使用阿伏帕星的蛋鸡养殖场中未分离出此类微生物。在送往医院厨房的肉鸡 carcasses 中也检测到了耐糖肽肠球菌。如通过诱导型 39-kDa 细胞质膜蛋白的检测以及聚合酶链反应扩增的 vanA 特异性 DNA 序列所表明的，耐药性由 vanA 基因决定。来自动物源的菌株的基因组 DNA 片段模式彼此不同，也与在医院和污水处理厂分离出的菌株的模式不同。这一发现表明 vanA 决定簇在不同生态来源的不同肠球菌菌株之间传播。 （注：carcasses 常见释义为“屠体” ，这里结合语境可能是指肉鸡的躯体部分 ）
