Klare I, Heier H, Claus H, Böhme G, Marin S, Seltmann G, Hakenbeck R, Antanassova V, Witte W
Robert Koch Institute, Wernigerode Branch, Germany.
Microb Drug Resist. 1995 Fall;1(3):265-72. doi: 10.1089/mdr.1995.1.265.
The occurrence and the further spread of high-level glycopeptide-resistant, vanA-positive Enterococcus faecium strains outside of hospitals have been investigated. We could isolate such bacteria directly from thawing liquids of commercially produced frozen poultry (chickens, turkeys; no further data on previous feeding with avoparcin were available). In 5 of 13 samples of raw minced meat of pigs originating from 13 different butcher's shops, glycopeptide-resistant E. faecium (VanA type) could be detected after overnight broth cultivation of these samples. No glycopeptide-resistant enterococci could be isolated from meat samples of chickens that were fed without avoparcin. VanA type E. faecium strains were also identified in 12 fecal samples recovered from 100 nonhospitalized humans in the rural area of Saxony-Anhalt federal county. These results suggest a possible role of the food chain in the spread of glycopeptide-resistant E. faecium. Molecular typing (macrorestriction and multilocus enzyme analysis) reveal a wide dissemination of the vanA gene among strains of different ecological origins.
对医院外高水平糖肽耐药、vanA阳性粪肠球菌菌株的出现及进一步传播情况进行了调查。我们能够直接从商业生产的冷冻家禽（鸡、火鸡；没有关于先前是否用阿伏帕星喂养的更多数据）的解冻液中分离出此类细菌。在来自13家不同肉店的13份猪绞肉生肉样本中，有5份样本经过夜肉汤培养后可检测到糖肽耐药粪肠球菌（VanA型）。在未用阿伏帕星喂养的鸡肉样本中未分离出糖肽耐药肠球菌。在从萨克森-安哈尔特联邦州农村地区100名非住院患者采集的12份粪便样本中也鉴定出了VanA型粪肠球菌菌株。这些结果表明食物链在糖肽耐药粪肠球菌传播中可能发挥作用。分子分型（宏观限制性酶切和多位点酶分析）显示vanA基因在不同生态来源的菌株中广泛传播。
