Kato N, Nakashima I, Ohta M
Jpn J Microbiol. 1976 Jun;20(3):173-81. doi: 10.1111/j.1348-0421.1976.tb00972.x.
The neutral fraction (neutral CPS-K) of Klebsiella pneumoniae capsular polysaccharide (CPS-K) from type 1, Kasuya strain, has already been reported as the active substance responsible for the strong adjuvant effect of CPS-K. The present results demonstrate that neutral CPS-K exhibits further common biological activities with lipopolysaccharide (LPS) isolated from Salmonella enteritidis. The intensity of the lethality in mice of neutral CPS-K by the intraperitoneal route is very similar to that of LPS. Its lethality for mice by the intravenous (i.v.) route is significantly stronger than that of LPS, because the degree of increase in the sensitivity to their lethality by i.v. challenge is smaller for LPS than for neutral CPS-K. The intensity of the pyrogenicity of neutral CPS-K in rabbits is approximately one-tenth of that of LPS as judged by the minimal pyrogenic doses and fever indices. The skin-preparatory potency of neutral CPS-K for the dermal Shwartzman phenomenon in rabbits is also approximately one-tenth of that of LPS compared on the basis of the minimal skin-preparatory doses. When injected i.v., neutral CPS-K exhibits a provocative effect on hemorrhagic reactions in skin sites prepared with neutral CPS-K or LPS.
来自1型茅野菌株的肺炎克雷伯菌荚膜多糖（CPS-K）的中性组分（中性CPS-K）已被报道为负责CPS-K强大佐剂效应的活性物质。目前的结果表明，中性CPS-K与从肠炎沙门氏菌分离的脂多糖（LPS）表现出进一步的共同生物学活性。中性CPS-K经腹腔途径对小鼠的致死强度与LPS非常相似。其经静脉（i.v.）途径对小鼠的致死性明显强于LPS，因为静脉内攻击对其致死性的敏感性增加程度，LPS比中性CPS-K小。根据最小致热剂量和发热指数判断，中性CPS-K在兔体内的致热强度约为LPS的十分之一。基于最小皮肤致敏剂量比较，中性CPS-K对兔皮肤施瓦茨曼现象的皮肤致敏效力也约为LPS的十分之一。静脉注射时，中性CPS-K对用中性CPS-K或LPS致敏的皮肤部位的出血反应具有激发作用。
