Ihalainen J, Taavitsainen M, Salmivaara T, Palotie A
Department of Clinical Chemistry, University of Helsinki, Finland.
J Clin Pathol. 1994 Dec;47(12):1082-4. doi: 10.1136/jcp.47.12.1082.
To improve the diagnostic value of fine needle aspiration biopsy of pancreatic lesions using a simple mutation detection method based on the polymerase chain reaction (PCR).
Fine needle aspirates from 21 suspected pancreatic lesions were analysed for K-ras codon 12 point mutations using solid phase minisequencing.
A point mutation in codon 12 of the K-ras gene was detected in 14 of 17 cases of pancreatic carcinoma. No false positive results were recorded. The concordance of the result with routine cytology was 78%. All patients diagnosed as having malignant disease on cytology also had a K-ras point mutation. Additional information on the presence of malignancy was obtained using molecular genetic analysis in two cases.
PCR based minisequencing is a promising method for the analysis of cytological material. K-ras point mutation analysis was modified to enable it to be carried out in a clinical laboratory. Advantages of the method include its simplicity and speed. Adequate sampling guidance is important but analysis can be performed even with small amounts of cellular material.
采用基于聚合酶链反应（PCR）的简单突变检测方法，提高胰腺病变细针穿刺活检的诊断价值。
使用固相微测序法分析21例疑似胰腺病变的细针抽吸物中K-ras密码子12点突变情况。
17例胰腺癌患者中有14例检测到K-ras基因密码子12点突变。未记录到假阳性结果。结果与常规细胞学的一致性为78%。所有经细胞学诊断为恶性疾病的患者也都有K-ras点突变。通过分子遗传学分析在2例患者中获得了有关恶性肿瘤存在的额外信息。
基于PCR的微测序是分析细胞学材料的一种有前景的方法。对K-ras点突变分析进行了改进，使其能够在临床实验室进行。该方法的优点包括简单和快速。充分的采样指导很重要，但即使是少量细胞材料也能进行分析。
